Introduction of Preparative Systems According to Purpose

Preparative HPLC

Shimadzu strives to improve the purity, speed, and cost efficiency of preparative HPLC. This page introduces the features of each configuration, to allow the customer to select the appropriate preparative system for the target fraction volume and sample type.

 

General Purpose Preparative System

LC-20AP solvent delivery unit

There are different pump options available for a variety of application requirements. In a large-scale preparative system, the LC-20AP solvent delivery unit is a powerful unit offering a maximum flow rate of 150 mL/min, suitable for automated continuous fractionation with 20 to 50 mm I.D. separation columns. For semi-prep applications, the LC-20AR delivers solvent flow rates up to 20 ml/min, to support separation on analytical columns up to 20 mm I.D. semi-preparative columns, while the LC-20AT pump can be used for semi-micro, analytical and semi-prep columns up to 10 mm I.D. and a flow of up to 10 ml/min.

Systems can be configured to suit a variety of purposes, from simple systems with manual injection and isocratic separation to automated systems with autosampler injection and gradient elution. The solvent delivery unit and amount of fraction collectors can be selected to suit the recovery volume. Additionally, the Shim-pack™ Scepter separation columns come in wide range of selectivities for a variety applications and provide excellent scalability from analytical to preparative scale.

Preparative System for Non-UV Absorptive Compounds

LH-40 and fraction collector FRC-40

If the target compounds or impurities do not absorb UV-light, preparative purification efficiency can be enhanced by adding a detector that detects analytes not based on the principle of light absorption, such as an ELSD (Evaporative Light Scattering Detector) or MS (Mass Spectrometer). Fractionation using a highly-selective MS trigger is advantageous, to achieve high purity of target components.

The liquid handler LH-40 and fraction collector FRC-40 can use up to four signal channels. Hence, analyzing compounds without a chromophore is not a limitation. In addition to UV or PDA, Nexera Prep can connect LCMS, RID and ELSD to detect and/or identify targets for purification.

Analytical and Preparative work in one system

Nexera Prep Analytical/Preparative System

The Nexera Prep Analytical/Preparative System permits smooth investigation of the fractionation conditions in both analytical and preparative scale. Simply operating a flow-line switching valve makes it easy to switch between the two flow lines. Using a single system to handle all processes from investigating separation conditions and sample loads with a conventional column to scaling-up reduces the consumption of mobile phase solvent. After sample collection, this system allows smooth and efficient switching to the analytical mode. Using the LH-40 Liquid Handler for re-injection enables preparative separation and fractionation, as well as re-analysis in analytical scale for confirmation of purity in the same system.

Preparative Recycling System

 
Preparative recycle system 

Components that are difficult to separate can be recovered at high purity level. In a closed valve recycling method, the eluate containing the target compounds after separation is recycled into the column, offering an equivalent separation capacity to that of a longer stationary phase. This is the optimal system for obtaining high separation efficiency at low cost.

Closed-Loop Recycle Flow Diagram

Components that are difficult to separate can be recovered at high purity level. In a closed valve recycling method, the eluate containing the target compounds after separation is recycled into the column, offering an equivalent separation capacity to that of a longer stationary phase. This is the optimal system for obtaining high separation efficiency at low cost.

Separation of Benzene and Mono-Deuteriated Benzene Substitute Column: STR ODS-II (250 x 20 mm I.D.) x 2

Increased Fractioning Rate Using Recycle Separation with Analytical ODS Column Column: STR ODS-II (150 x 6 mm I.D.) Flowrate: 2 mL/min, sec-/iso-/n-butylbenzene

Ultra-Fast Preparative and Purification LC

This solution provides fast recovery of highly purified target compounds in a simple workflow, by automation of fractionation, concentration, purification and recovery. Using a combination of preparative LC and trapping columns this system offers automated preparative isolation of target compounds in 3 steps:

  1. Separation of target compounds by preparative LC before introduction into trapping columns
  2. Replacement of solvent in trapping columns with ultrapure water
  3. Elution of target compounds from trapping columns by organic

The special Purification Solution software is equipped with a peak tracking function, for confirmation of target peaks at a glance:

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